绞股蓝提取液对光老化模型小鼠皮肤组织中SOD活性MDA和HYP含量影响的实验研究

To InvestigateG.Pentaphyllum's Influenceon the Content of HYP and MDA as Well as the Activity of SOD in the Skin of Hairless Photo Aging Mice

目的:研究内服绞股蓝对紫外线照射引起的无毛小鼠皮肤光老化模型的影响。方法:昆明无毛小鼠随机分成5组,正常组(A)、模型对照灌胃组(B)、低剂量灌胃组(C)、中剂量灌胃组(E)、高剂量灌胃组(F)。模拟UV长期照射,造成皮肤光老化模型。给药组照射同时灌胃绞股蓝提取液。采用病理组织切片制片技术,HE染色以40倍光镜观察真皮结构改变,并以生化分析方法测定SOD活性,HYP及MDA含量。结果:绞股蓝C、D、E组对SOD活性均有提高作用(P<0.05)。D、E组可提高羟脯氨酸含量,降低MDA含量。结论:绞股蓝提取液具有拮抗紫外线照射引起的无毛小鼠皮肤光老化的作用。

Objective： Study to take orally to C. pentaphyllum whether in the skin of hairless photoageing mice model has the function or not that project light upon to cause to the ultraviolet rays. Methods： Healthy KM hairless mice were divided into seven groups randomly and garages respectively,that is negative Control Group（A） ,Model Group（B） ,Model ＋ Low- G. Pentaphyllum group （ C）, Model ＋ Medium - G. Pentaphyllum Group （ D）, Model ＋ High - G. Pentaphyllum Group （E）. The mice in B, C, D, and E group were irradiated to simulate skin photoageing by long- term ultraviolet irradiation, at the same time ,those in C ,D,and E were given G. Pentaphyllum. Staining skin with HE, Under 40 times light microscope, the structure of dermal were observed. While the changes of the contents of dermal hydroxyproline （HYP） and Malondialdehyde（MDA） ,as well as the activity of Superoxide Dismutase（SOD） were measured by biochemical test method in each group of the experiment. Results ：The contents of SOD in dermal in group C, D, E were increased that in group B, Compared with the group B,the content of HYP was increased,while the content of MDA was reduced in group C,D,E（ P 〈0.05）The skin biopsies appear photoageing look. Conclusions： G. Pentaphyllum has the protective effect on the skin of the photoageing hairless mice with ultraviolet irradiation.