HaNPV病毒对寄主昆虫的持续作用研究

Studies on Sustainable Effects for Helicoverpa armigera Nuclear Polyhedrosis Virus to Host Insects

用不同浓度的HaNPV病毒感染3龄棉铃虫幼虫并分别收集不同处理的存活试虫进行室内传代饲养。结果表明,病毒对寄主昆虫不仅具有直接致死作用,而且对蛹质量和化蛹均有明显的影响,尤其是对亲代和子一代的寄主昆虫。同时,通过PCR技术,从子一代的幼虫和蛹中成功检测到该病毒,从而在分子水平上证明了病毒能够经过亲代传递到子代的种群中。这对病毒的高效利用和害虫的持续控制具有重要的理论和实践意义。

Third instars larvae of cotton bollworm, Helicoverpa armigera, were infected with different concentrations of Helicoverpa arrnigera nucleopolyhedrovirus （HaNPV）. Survivors were collected respectively and reared in laboratory room. Mortality rate of the larvae, average weight of the single pupa and pupation rate were observed in parental generation and successive filial generations. The results showed that the virus not only led to death of larvae in parental and folial generation, but also impacted the average weight of the single pupa and pupation rate, especially to the parental and the first folial generation. The experimental data indicated that the virus could weaken the host in folial generations, which could control populations of the host for a long time. Meanwhile, pelymerase chain reaction （PCR） amplification of polyhedrin gene sequences demonstrated that the virus was present in eggs and pupae whose parents were infected. This method might provide a better understanding of nucleopolyhedrovirus which could transmit from parental generation to folial generation. The research would play an important role in utilizing the virus effectively to control the pest persistently.