摘要
In this study,an efficient regeneration system of Broussonetia papyrifera was established.Using leaf disks and petiole fragments as explants,MS media supplemented with different concentrations of BA and NAA were investigated for shoot induction.Then regenerated shoots were transferred to MS medium containing 0～0.6 mg·L-1 NAA to induce roots.A BA/NAA combination of 2.5/0.1(mg·L-1) produced the highest percentage of regeneration with petiole explants and of 1.5/ 0.05(mg·L-1) with leaf explants.The optimal medium for rooting was MS medium supplemented with 0.05 mg·L-1 NAA.
In this study, an efficient regeneration system of Broussonetia papyrifera was established. Using leaf disks and petiole fragments as explants, MS media supplemented with different concentrations of BA and NAA were investigated for shoot induction. Then regenerated shoots were transferred to MS medium containing 0 -0.6 mg·L^-1 NAA to induce roots. A BA/NAA combination of 2.5/0.1 （ mg ·L^-1 ） produced the highest percentage of regeneration with petiole explants and of 1.5/0. 05 （mg·L^-1） with leaf explants. The optimal medium for rooting was MS medium supplemented with 0.05 mg·L^-1 NAA.
出处
《林业科学研究》
CSCD
北大核心
2006年第2期253-256,共4页
Forest Research
基金
国家自然科学基金(NNSFC:30471411)
关键词
光叶楮
组织培养
快繁
paper mulberry
tissue culture
plant regeneration
