转染rhscIL-12对人树突状细胞功能的影响

Effect of rhscIL-12 gene transfection on function of human dendritic cells

目的观察rhscIL12基因转染的人树突状细胞(dendriticcells,DCs)功能的改变。方法构建rhscIL12和逆转录病毒载体的重组体,脂质体介导转染人外周血来源DCs,以流式细胞仪分析细胞表型改变,ELISA检测IL4、IL12和IFNγ的水平。结果rhscIL12转染DCs后可进一步上调DCs表面分子的表达,IL12的分泌量明显提高;在混合淋巴细胞反应中,Th1型细胞因子IFNγ的水平明显上升,Th2型细胞因子IL4显著下降;随着IL12的分泌量的增加,IFNγ含量明显增加,二者存在着显著正相关关系(r=0.955678,P<0.01)。结论rhscIL12体外转染DCs能上调DCs表面分子表达,诱导Th0向Th1分化,增强机体抗肿瘤免疫效应。

Objective To study the function changes of human dendritic cells after rhscIL-12 gene transfection. Methods The recombinant of rhscIL-12 gene and retroviral vector were constructed and transfected into dendritic cells from human peripheral blood by lipofection. The phenotype changes of DCs were analyzed by fluorescence-activated cell sorter （FACS） and IL-4, IL-12 and IFN-γlevels were detected using ELASA. ReSUitS The expression of DCs surface molecules and IL-12 level were significantly increased after rhscIL-12 gene transfection into human dendritic cells. In the mixed lymphocyte reaction, the IFN-γlevel increased and IL-4 decreased significantly. With the IL-12 level rising, the IFN-γlevel increased significantly, suggesting a positve correlation between IL-12 and IFN-γ（ r = 0. 955 678, P 〈 0. 001 ）. Conclusion rhscIL-12 gene transfection into human DCs can upregnlate the surface molecules, induce Th0 cells differentiate to Th1, and en- hance the immune response to tumor.