摘要
目的为糖皮质激素(GCS)对神经细胞的作用提供形态学资料。方法30只SD大鼠随机分为:对照组、单纯甲醛组、地塞米松预处理组、RU486预处理组、RU486+地塞米松预处理组,甲醛刺激后采用动物行为学方法、免疫组织化学法、神经细胞染色和形态计量学方法进行观察。结果在大鼠后爪掌面皮下注射稀释甲醛20μl可造成注射处急性持续性炎症。1h后,同侧脊髓腰膨大背角浅层。双侧中缝大核、丘脑、大脑皮层Fos免疫阳性神经元(FLI)总数明显上升,对侧脊髓内少量散在分布。用地塞米松预处理2h后可使相应部位FLI神经元数明显降低,具有剂量依赖性。外周水肿。疼痛表现与同侧背角浅层FLI细胞数呈正相关。用糖皮质激素受体阻断剂RU486可部分反转GCS的效应。结论伤害性刺激可引起中枢神经系统c-fos广泛表达。GCS可降低脊髓后角神经元的兴奋性。
Objective To supply morphological data for exploring the actions of glucocorticoids （GCS） on neurons. Methods 30 SD rats were divided in groups as following： contral group, formalin group, GCS group, RU486 group and RU486＋ GCS group. After formalin stimulation, animal behavior observation, immunohistoehemical neurons staining and morphometry were used in this study. Results The Sprague-Dawley rats, injected with 20 ml of 2.5% formalin subcutaneously into the right hind paw, occurred acute continuous inflammation near area of the injection. After one hour, the total number of Fos-like immunoreactive （Fos-LI） neurons in the ipsilateral lumbar spinal cord, bilateral major rapheal nuclie, thalamus, cerebral cortex was significantly increased. Fos-LI was sparse in the contralateral spinal cord. Pre-administered dexamethasone for 2 hours reduced the total number of Fos-LI neurons more significantly in the same areas in dose-dependent manner. The total number of c-Fos-LI neurons in the ipsilateral lumbar spinal cord were positively correlated with the peripheral oedema and pain. The effects of GCS can be partly reversed by glucoeortlcoid receptor blocking agent RU486. Conclusion The expression of c-los in the central nervous system is involved in response to a peripheral noxious stimuli. GCS may depress the excitement of dorsal horn of spinal cord.
出处
《解剖学研究》
CAS
2006年第1期15-17,F0004,共4页
Anatomy Research
