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保罗样激酶1mRNA及蛋白质在胃癌中的表达意义

Significance of polo like kinase1 and protein expression in gastric carcinomas
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摘要 目的探讨保罗样激酶1(plk1)在胃癌组织中mRNA及蛋白质的表达,并探讨其表达水平与临床病理指标的关系。方法采用实时定量多聚酶链反应及免疫印迹(W estern blot)分别检测了6 0例胃癌患者新鲜切除胃癌组织及其对应正常胃粘膜组织中plk1 mRNA及蛋白质的表达。结果胃癌组织中plk1mRNA及蛋白质水平均明显高于正常组织(P<0.0 5,P<0.0 1);并且其水平与胃癌的分化程度和浸润深度有关(P<0.0 5);蛋白质表达水平与胃癌的分化有关(P<0.0 5)。结论plk1的过表达可能在胃癌发生发展中起促进作用;其表达程度可望作为胃癌的某些生物学行为新的判定指标。 Objective To study the significance of polo like kinase 1 （ plk 1 ） mRNA and protein expression in gastric cancer tissues and the relationship between the plk I mRNA and protein expression level in gastric cancer tissues and the elinicopathological parameters. Methods Plkl mRNA and protein level were respectively measured by real- time PCR and Western blot in fresh tumor tissues and the normal gistric mucosa from 60 patients With gastric cancer. Results plk 1 mRNA and protein expression level in gastric cancer tissues was significantly higher than that in normal gastric tissues （ P 〈 0. 05 , P 〈 0.01 ） . plk 1 mRNA expression level in gastric cancer tissues was closely related to degree of tumor differentiation and depth of invasion（ P 〈0.05 ）, and plkl protein level was closely related to tumor differentiation （ P 〈 0.05 ）. Conclusions The overexpression of plk 1 may promote carcinogenesis and the development of gastric cancer, and plk 1 can be a novel marker for determing certain biological behaviours of gastric cancer.
作者 兰斌 陈雪华 刘炳亚 瞿颖 蔡劬 朱正纲
机构地区 上海交通大学医学院附属瑞金医院外科上海消化外科研究所
出处 《中国普通外科杂志》 CAS CSCD 2006年第2期98-101,共4页 China Journal of General Surgery
基金 国家973重点基础研究发展规划基金资助项目(2002CB713700) 福建医科大学科学研究发展基金资助项目(FJGXY040727)
关键词 胃肿瘤/病理生理学 保罗样激酶1 Stomach Neoplasms / physiopathol plk 1
分类号 R735.2 [医药卫生—肿瘤]
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参考文献14

  • 1Liu X,Erikson RL.Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk 1 by siRNA [J].Proc Natl Acad Sci.2002,99(13):8672-8676.
  • 2Smith MR,Wilson ML,Hamanaka R,et al.Malignant transformation of mammalian cells initiated by constitutive expression of the polo-like kinase [J].Biochem Biophys Res Commun [J].1997,234(2):397-405.
  • 3Takahashi T,Sano B,Nagata T,et al.Polo-like kinase 1 (PLK1) is overexpressed in primary colorectal cancers [J].Cancer Sci.2003,94(2):148-152.
  • 4Weichert W,Denkert C,Schmidt M,et al.Polo-like kinase isoform expression is a prognostic factor in ovarian carcinoma [J].Br J Cancer.2004,90(4):815-821.
  • 5Tokumitsu Y,Mori M,Tanaka S,et al.Prognostic significance of polo-like kinase expression in esophageal carcinoma [J].Int J Oncol.1999,15(4):687-692.
  • 6Wolf G,Elez R,Doermer A,et al.Prognostic significance of polo-like kinase (PLK) expression in non-small cell lung cancer[J].Oncogene,1997,14(5):543-549.
  • 7Knecht R,Elez R,Oechler M,et al.Prognostic significance of polo-like kinase (PLK) expression in squamous cell carcinomas of the head and neck[J].Cancer Res,1999,59(12):2794-2797.
  • 8Kneisel L,Strebhardt K,Bernd A,et al.Expression of polo like kinase (PLK1) in thin melanomas:A novel marker of metastatic disease [J].J Cutan Pathol,2002,29 (6):354-358.
  • 9Dietzmann K,Kirches E,yon Bossanyi,et al.Increased human polo-like kinase-1 expression in gliomas [J].J Neurooncol,2001,53(1):1-11.
  • 10Wolf G,Hildenbrand R,Schwar C,et al.Polo-like kinase:A novel marker of proliferation:Correlation with estrogen-receptor expression in human breast cancer [J].Pathol Res Pract,2000,196(11):753-759.

二级参考文献23

  • 1[5]Golsteyn RM, Mundt KE, Fry AM, et al. Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function. J Cell Biol, 1995, 129(6):1 617-28.
  • 2[6]Taniguchi E, Toyoshima-Morimoto F, Nishida E. Nuclear translocation of plk1 mediated by its bipartite nuclear localization signal. J Biol Chem, 2002, 277(50):48 884-8.
  • 3[7]Roshak AK, Capper EA, Imburgia C, et al. The human polo-like kinase, PLK, regulates cdc2/cyclin B through phosphorylation and activation of the cdc25C phosphatase. Cell Signal, 2000, 12(6):405-11.
  • 4[8]Jackman M, Lindon C, Nigg EA, et al. Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol, 2003, 5(2):143-8.
  • 5[9]Chase D, Serafinas C, Ashcroft N, et al. The polo-like kinase PLK-1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans. Genesis, 2000, 26(1):26-41.
  • 6[10]Liu X, Erikson RL.Polo-like kinase (Plk)1 depletion induces apoptosis in cancer cells. Proc Natl Acad Sci USA, 2003, 100(10):5 789-94.
  • 7[11]Smits VA, Klompmaker R, Arnaud L, et al. Polo-like kinase-1 is a target of the DNA damage checkpoint. Nat Cell Biol, 2000, 2(9):672-6.
  • 8[12]Liu X, Erikson RL. Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk1 by siRNA. Proc Natl Acad Sci USA, 2002, 99(13): 8 672-6.
  • 9[13]Sumara I, Vorlaufer E, Stukenberg PT, et al. The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase. Mol Cell, 2002, 9(3):515-25.
  • 10[14]Neef R, Preisinger C, Sutcliffe J, et al. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J Cell Biol, 2003, 162(5):863-75.

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中国普通外科杂志
2006年 第2期

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