RT-PCR检测超声辐照后人早孕绒毛组织中Caspase-3mRNA的表达

Detection of the Expression of Casepase-3 mRNA in Human Villi after Diagnostic Ultrasound Irradiation by RT-PCR

目的:探讨诊断性超声对早孕胚胎组织安全性问题。方法:选取健康早孕而自愿要求终止妊娠的妇女66例,随机分组,不同的超声辐照方式和不同辐照时间后12-24h行人工流产取绒毛组织,用半定量RT-PCR方法分析绒毛组织中Caspase-3mRNA的表达。结果:绒毛组织Caspase-3mRNA表达,腹部超声5min组与对照组(未行超声辐射组)相比无统计学差异(P>0.05),而10min组、15min组与对照组比较有统计学意义(P<0.05),但此两组间无统计学差异(P>0.05);阴道超声5min、10min、15min组与对照组比较均有显著的统计学意义(P<0.01),且各组间比较也有差异(P<0.01);同一时间组内阴道超声与腹部超声比较均有统计学差异(P<0.05)。结论:诊断性超声经腹部5min以内可以认为是安全的,从近期的生物学效应角度看,经阴道超声在5min时就可能存在损伤效应。

Objective： To deeply the safety of diagnostic ultrasound on embryo during early pregnancy. Methods： Sixty-six healthy early pregnant women who had accepted labor abortion were randomly divided into different groups. The villi after accepting different ways and time of ultrasound irradiation were taken out immediately after labor abortion, and the expression of Caspase-3 mRNA in human villi was analysed by semi-quantitative RTopCR. Results： In transabdominal groups, except for 5 min group, 10 min group and 15 min group showed great significance compared with control group （P〈0.05）,the difference between the latter groups were not existed （P〉0.05）. Each group in transvaginal groups had great significance compared with control group（P〈0.01）. Conclusion： The bio-effect of transabdominal ultrasound below 5 min on early pregnant embryo is safe. From the view of instant and short-term on human villi, this bio-effect effect may be not safe when exposed time is 5 min by transvaginal ultrasound.