新型骨替代材料-珍珠层的生物相容性研究

Experimental study on biocompatibility of nacre

[目的]研究珍珠层的生物相容性。[方法]在体外培养的第3代成骨细胞中分别加入珍珠层(实验组)、橡胶(对照组)的材料浸提液,观察细胞形态、细胞膜和超微结构的变化;通过B rdU核掺入法检测细胞增殖情况、通过MTT法检测成骨细胞代谢活性。[结果]实验组细胞形态及细胞结构良好、细胞膜完整;对照组中细胞形态发生改变,细胞膜及胞浆内细胞器均有破坏;B rdU及MTT检测结果显示珍珠层对人成骨细胞的增殖和代谢活性无阻滞作用。[结论]珍珠层具有良好的生物相容性,可作为骨替代材料应用于骨缺损修复。

[ Objective] To study the biocompatibility of nacre on cultured human osteoblasts in vitro. [ Method] The iliac bone of human were added with collagenase-trypsin, a lot of osteoblasts were released out. Then the osteoblasts were purified and cultured. Subsequently, the 3rd generation cultured human osteoblasts were inoculated with the extract of three materials. nacre, hydroxyapatite, and robber. The appearance of osteoblast was observed under the phase contrast microscope. Microstrocture of the osteoblast was observed under transmission electron microscope. Additionally, to determine whether these three materials affect cell proliferation, the numbers of BrdU-labeled cells were examined at three groups. MTr assay was tested to observe the viability of human osteoblasts. [ Result] Under the phase contrast microscope, the appearance of the osteoblasts in two groups were spindle or triangle like, and black nodes were seen in confluent cell layer 19 days after cultured. The observation of electron microscopy showed that the nucleus of the osteoblasts in two groups were big and round, and there were a large number of mitochondrion and rough endoplasmic reticulum in two groups. Stereological analyses of the numbers of BrdU-labeled cells revealed that females produced more cells than males in the dentate gyros but not in the subventricular zone. [ Conclusion] The nacre have no harmful effect to character of biology of human osteoblasts in vitro.