纤维粘连蛋白EDA^+片段在退变椎间盘中的表达及其意义

Expression of EDA^+ fibronectin splicing variants in the degenerated lumbar disc

[目的]检测退变椎间盘内EDA+Fn的表达,探讨损伤在椎间盘退变中的作用。[方法]收集人腰椎间盘退变临床手术标本,经病理证实均发生退变,采用逆转录聚合酶链反应(RT-PCR)技术扩增目标条带。[结果]正常椎间盘内没有EDA+Fn的表达,而退变椎间盘内均有EDA+Fn的表达;中重度退变者EDA+Fn的表达较轻度退变者为多(轻中度组间P=0.016<0.05;轻重度组间P=0.007<0.05),中重度之间则没有明显差别(P≥0.501)。[结论]EDA+Fn在退变椎间盘内有较高表达,直接证明损伤是椎间盘退变的重要的致病因素之一。

[ Objective] To study the relationship between trauma and the lumbar disc degeneration and to detect the expression of the fibronectin splicing variants in the degenerated lumbar intervertebral disc, and study the expression of EDA. [ Method] The human lumbar intervertebral disc tissues were collected to extract total RNA which was then amplified by RTPCR technique. [ Result] EDA^＋ Fn of normal lumbar intervertebral disc were not expressed, whereas EDA^＋ Fn of degenera- ted lumbar intervertebral disc were expressed abundantly. The defference between different groups has statistical significance. [ Conclusion] EDA^＋ Fn has very strongly expression in the lumbar degenerated intervertebral disc, and the trauma is one of prerequisite factors in the disc degenerated process probablely.