积雪草甙和苦参碱对皮肤瘢痕成纤维细胞生长及胶原合成影响的研究

Research of Effects on Scars Fibroblasts Proliferation and Collagen Synthesis by Chinese Herbs Asiaticoside and Matrine

目的探讨积雪草甙、苦参碱对皮肤瘢痕成纤维细胞生长及胶原合成的影响,并对两者的抗瘢痕作用强度进行比较。方法体外培养瘢痕疙瘩成纤维细胞,分别加入不同浓度的积雪草甙、苦参碱,通过倒置显微镜观察细胞形态、MTT法测定细胞活力、流式细胞仪检测细胞周期改变、氯胺T法测定羟脯氨酸含量、乳酸脱氢酶检测细胞毒作用,分别观测两种中药对瘢痕成纤维细胞生长及胶原合成的影响。结果与空白对照组相比,实验组中积雪草甙为0.5g/L、苦参碱为1.00g/L后成纤维细胞数量均明显减少;羟脯氨酸含量降低(P<0.05);细胞处于G2-M期比例增加(P<0.05);当积雪草甙浓度低于5g/L时乳酸脱氢酶含量在正常范围内。结论在一定浓度下,积雪草甙和苦参碱均能抑制皮肤瘢痕成纤维细胞生长及胶原合成,积雪草甙的抗瘢痕作用强于苦参碱。

Objective To research the changes in fibroblasts growth and collagen synthesis by adding Chinese herbs asiaticoside and matrine, and then compare their ability of inhibition. Methods All of the experiments were detected in vitro culture system. Fibroblasts were cultivated from keloid tissue. They were divided into 3 groups： one control group and two trial groups. Asiaticoside and matrine were added into each culture medium for fibroblasts. Then the cells proliferation and collagen synthesis was evaluated by microscope observation, MTT assay, flow cytometry, chloramines T measure for hydroxyproline and lactate dehydrogenase activity determination. Results Comparing the changing of control group and trial groups（0.5 g/L asiaticoside and 1.00 g/L matrine）： cells proliferation was reduced （P〈0.001）, collagen synthesis was suppressed（P〈0.05） and ratio of G2-M phase in cell cycle was increased. Lactate dehydrogenase contents were inside reference value range in matrine group, while they exceeded for the concentration of 5.00 g/L in asiaticoside group. Conclusion With effective concentration, scar fibroblasts proliferation and collagen synthesis can be inhibited by asiaticoside and matrine. Ability of inhibition of asiaticoside prevails is better than that of matrine.