常用治疗银屑病的中药对肿瘤坏死因子-α刺激后角质形成细胞生长及分泌白介素-8的影响

Effects of 8 Kinds of Chinese Herbs on the Growth and Interleukin-8 Production of Keratinocytes Stimulated by Tumor Necrosis Factor-α

目的研究常用中药治疗银屑病的作用机制。方法采用体外细胞培养技术,观察常用于治疗银屑病的8味中药提取液对角质形成细胞生长及肿瘤坏死因子-α(TNF-α)刺激后角质形成细胞分泌细胞因子白介素-8(IL-8)的影响。结果多数中药表现出抑制角质形成细胞增殖的作用,其中牡丹皮、赤芍、金银花、拳参、全蝎、苦参还表现出拮抗TNF-α刺激后角质形成细胞分泌IL-8的作用。结论初步证明几种常用于治疗银屑病的中药具有抑制TNF-α刺激后角质形成细胞增殖及分泌IL-8作用,从细胞生物学水平探讨了中药抗银屑病作用机制。

Objective To study the mechanism of Chinese herbs on treating psoriasis（PS）. Methods Extractions that got from 8 kinds of Chinese herbs of curing psoriasis（PS）, and then the culture of human transformation keratinocytes （KCs） and tumor necrosis factor-α（TNF-α） was used to release interleukin-8 （IL-8） and the effects of these extracts on the cells were observed. Results Most of the herbs that we tested could restrain the proliferation of KCs. And some of them, such as CORTEX MOUTAN, RADIX PAEONIAE RUBRA, RHIZOMA BISTORTAE, SCORPIO, RADIX SOPHORAE FLAVESCENTIS, FLOS LONICERAE could also lower the IL-8 lever induced by TNF-α. Conclusion The results indicate that the effects of some Chinese herbs on treating PS are probably mediated by inhibiting KCs＇ proliferation and blocking IL-8 production.