玫瑰糠疹与人类疱疹病毒-7型关系的研究

Relationship Between Pityriasis Rosea and Human Herpes Virus 7

目的探讨玫瑰糠疹(PR)与人类疱疹病毒-7型(HHV-7)的关系。方法采用巢式PCR检测了22例PR急性期患者的血浆、外周血单核细胞(PBMC)、皮损、唾液、尿液,14例恢复期患者的唾液、血浆、外周血单核细胞,14例正常人的唾液、血浆、外周血单核细胞中的HHV-7特异性序列。结果急性期唾液与单核细胞中HHV-7DNA检出率(95.5%,45.4%)明显高于正常人(64.3%,21.4%),唾液中HHV-7DNA检出率亦明显高于恢复期(57.1%),但单核细胞中HHV-7DNA检出率与恢复期(28.6%)无明显差异。8例皮损组织(36.4%)检测到HHV-7DNA。1例血浆中检测到HHV-7DNA(4.54%),其外周血单核细胞、皮损、唾液中亦均检测到HHV-7DNA,其恢复期唾液、单核细胞中HHV-7DNA仍可检测到。恢复期HHV-7DNA在唾液和单核细胞中的检出率与正常人无明显差别。结论有一部分玫瑰糠疹的发生可能与潜伏的HHV-7活化感染有关。

Objective To explore the relationship between pityriasis rosea and human herpes virus 7 （HHV-7）. Methods The specific HHV-7 sequences were examined with a nested polymerase chain reaetion（PCR） procedure in the lesional skin, plasma, peripheral blood mononuclear cells （PBMC）, saliva, and urine samples of 22 cases of acute pityriasis rosea patients as well as in the saliva, plasma and PBMC of 14 convalescent pityriasis rosea patients. The saliva, plasma and PBMC samples of 14 healthy subjects were used as controls. Results Frequencies of HHV-7 specific DNA sequence detection in the saliva and PBMC （95.5% and 45.4%respectively） and 22 acute patients were higher than that of 14 normal controls （64.3% and 21.4% respectively）. The positive rate in the saliva of acute patients was also higher than that of the controls（57.1%）, but it was not higher than that of the controls （28.6%）in the PBMC. The HHV-7 specific DNA sequence was detected in 8 cases skin lesional （36.4%） of acute patients. Among these positive patients, 1 cases were positive for HHV-7 DNA sequence in the plasma and also in the PBMC, lesional skin and saliva. And in this patient the HHV-7 DNA sequence could be detected in the saliva and PBMC during convalescence. The HHV-7 DNA sequence in the saliva and PBMC of 14 convalescent pityriasis rosea patients had no significantly differences with that of the controls. Conclusion The occurrence of some pityriasis rosea may relate to the activation of HHV-7.