一种高效感染血液细胞的新型靶向性腺病毒载体的构建

Construction of a Novel Retargeted Adenoviral Vector with High Gene Transfer Efficiency into Hematopoietic Cells

人C组5型腺病毒(Ad5)载体能够有效感染上皮来源的细胞,但对造血细胞的感染效率很低,限制了其在造血调控基础研究以及血液病基因治疗中的应用。为了建立高效感染血液细胞的新型靶向性腺病毒载体系统,对5型腺病毒载体的纤维顶球进行了改造,以AdEasy系统为基础,应用递归PCR的方法人工合成人B组11p型腺病毒的部分纤维(fiber)基因,采用一系列分子生物学方法将其替换AdEasy骨架质粒中的人5型腺病毒的fiber基因,得到新的腺病毒骨架质粒命名为pAdEasy-1/F11p,应用带有GFP报告基因的穿梭质粒pShuttle-GFP与AdEasy-1/F11p腺病毒DNA在BJ5183细菌内重组得到重组腺病毒质粒,将其转染293细胞获得重组腺病毒,命名为Ad5F11p-GFP。以Ad5-GFP作对照,同时感染K562、U937等白血病细胞系,流式细胞仪检测GFP的表达。初步检测结果显示:在10MOI时,Ad5F11p-GFP能够有效感染K562、U937等白血病细胞系,感染细胞效率>90%,对照Ad5-GFP感染细胞效率<30%,这表明改建后的腺病毒AdEasy-1/F11p可以高效介导基因转移到血液细胞,是一种很好的血液细胞靶向性腺病毒载体。

The adenovirus subgenus C serotypes 5 （Ad5） vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells. For feasible generation of fiber-retargeted adenoviral vectors, we have modified the versatile AdEasy system with a chimeric fiber gene encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. An Ad5-based vector encoding the green fluorescent protein （GFP） gene under the control of the CMV promoter with Ad11p fiber receptor specificity was generated （Ad5F11p-GFP）. The Ad5F11p-GFP vector-mediated gene transfer efficiency for some committed hematopoietic cell lines such as myeloblasts （K562） and monocytes （U937） was evaluated using flow cytometry and compared to that of Ad5-GFP, which also encodes the GFP gene under the control of the CMV promoter. The results showed that these cell lines were superiorly transduced by the Ad5F11p-GFP vector at the same MOI （multiplicity of infection, MOI） compared with the Ad5-GFP vector, more than 90% tested cells were infected by Ad5F11p-GFP adenov, irus while less than 30% cells were infected by Ad5-GFP at 10 MOI.