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肺腺癌及其转移淋巴结的基因表达谱研究

Gene Expression Profile of Pulmonary Adenocarcinoma and Its Metastatic Lymph Nodes
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摘要 背景与目的利用基因芯片技术研究肺腺癌组织及其转移淋巴结组织基因表达差异。材料与方法分别抽取3例肺腺癌组织、转移淋巴结组织的总RNA,通过逆转录合成cDNA,以两种荧光cy5和cy3标记,制备cDNA探针,并与含有1152个人类全长基因的cDNA基因芯片进行杂交。以ScanArray4000荧光扫描仪扫描芯片上两种荧光信号,用计算机处理和分析。结果3例肺腺癌组织和转移淋巴结组织标本中,8个基因有共同表达差异,其中上调基因5个,下调基因3个。结论肺腺癌组织和转移淋巴结组织之间存在着基因表达差异,两者比较共表达差异的8个基因可能与肺腺癌的发展、转移有关。 BACKGROUND ＆ AIM： To investigate the differences of gene expression profile among the pulmonary adenocarcinoma and metastatic lymph node tissues by means of cDNA microarTay. MATERIAL AND METHODS： Total RNA was extracted from pulmonary adenocarcinoma and metastatic lymph node tissues in order to get purified mRNA which was retro-transcribed into cDNA. As the probes, the cDNA was labeled with Cy5 and Cy3 fluorescence, then hybridized with the whole human gene chip, The signal image was obtained by Scan Array 4000 fluorescence scanner and analyzed with computer software. RESULTS：8 genes in 3 subjects with pulmonary adenocarcinoma and metastatic lymph node tissues were identified with common differences in gene expression level, including 5 up-regulated and 3 down-regulated genes. CONCLUSION： The expression levels of 8 genes found both in pulmonary adenocarcinoma and metastatic lymph node tissues were probably related to the development and metastasis of lung cancer.
作者 张薇 石玉枝 霍建民
机构地区 哈尔滨医科大学第一临床医学院
出处 《癌变．畸变．突变》 CAS CSCD 2006年第2期105-107,共3页 Carcinogenesis,Teratogenesis & Mutagenesis
基金 黑龙江省自然科学基金(No.D200523)
关键词 肺腺癌 转移淋巴结 基因表达谱 pulmonary adenocarcinoma metastatic lymph nodes gene expression profile
分类号 Q132.7 [生物学—普通生物学] Q754 [生物学—分子生物学]
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参考文献13

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癌变．畸变．突变
2006年 第2期

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