番茄红素对高脂兔血管脂质过氧化损伤的影响及机制探讨

Study of mechanisms and effects of lycopene on lipid peroxidation injure in hyperlipemia rabbits

目的:观察番茄红素对高脂喂饲所致家兔主动脉脂质过氧化损伤的影响。方法:采用高脂喂饲模型,将30只新西兰家兔随机分为3组:对照组、高脂模型组和番茄红素组,分别给予普通饲料和高脂饲料喂养,番茄红素组另加用番茄红素;于实验开始前1 d和开始后第24、、6、8周末取空腹血测定血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇、血清超氧化物歧化酶活性、丙二醛含量、血清一氧化氮含量和一氧化氮合酶活性。实验结束后,苏丹Ⅳ染色和HE染色观察主动脉血管病理变化。结果:与高脂模型组相比,番茄红素组血清甘油三酯含量减低;血清超氧化物歧化酶活性明显增加;血清丙二醛的含量减少;一氧化氮含量增加;主动脉脂质斑块面积减少。结论:番茄红素对兔主动脉脂质过氧化损伤具有较好的保护作用,能减轻高脂兔动脉粥样硬化病变形成。降甘油三酯,抗脂质过氧化损伤及保护血管内皮功能可能是番茄红素产生抗动脉粥样硬化作用的重要机制。

AIM： To continuously observe the protective effects of lycopene on lipid peroxidation injure in hyperlipemia rabbits and to study the possible mechanisms. MEDHODS： 30 New Zealand rabbits were randomly divided into three groups. They were individually housed in metal cages. Throughout the experimental period, they were given restricted amounts of food （80-100g·h^-1 per day）. Control group was fed with normal diet; Modelgroup was fed with 1% cholesterol, 10% Lard and 89% normal diet; Lycopene group was fed with 1% cholesterol, 10% lard and normal diet plus 6% lycopene （60mg·kg^- 1·d^-1） ; At the time of the first day, the second, 4th,6th and 8th week, Blood samples were drawn from ear edge vein of rabbits. Total cholesterol （TC）, Triglyceride （TG） and High density lipoprotein cholesterol （HDL-C）, Superoxide dismutase （SOD） activity and Ma-leic dialdehyde （MDA） content of serum were detected. The level of serum nitric oxide synthases （NOS） activity and Nitric oxide （NO） were determined. At the end of the study, the plaque areas were measured. SPSS 10.0 software was used to evaluate the differences between three groups. RESULTS： Compared with Model group, lycopene could reduce the levels of serum TG and MDA, increase serum SOD activity, increase serum NO, dimin-ish lipid plaque areas significantly. CONCLUSION： Lycopene has antiatherogenic effect. The possible mechanisms may be that lycopene can reduce TG, decrease lipid peroxidation injure and protect vascular endothelium in hyperlipemia rabbits.