陆地棉EST-SSRs在向日葵中的通用性研究

Transferability of Upland Cotton EST-SSRs to Sunflower

利用陆地棉EST—SSRs（Expressed sequence tags—simple sequence repeats）在陆地棉和向日葵品种中进行了通用性分析。利用本实验室合成的1500对陆地棉EST—SSRs在4个陆地棉品种中进行了扩增，有效扩增比率为95％，扩增产物符合预期大小（50～500bp）；每对引物可以检测到1～6个数目不等的片段（allele），平均约为2．7个；每2个陆地棉之间大约有7％～9％的多态性。从1500对陆地棉EST—SSRs引物中选取400对引物在向日葵中进行了扩增，有效扩增比率为63％．每对引物可以检测到1～6个数日不等的片段，平均为1．7个，片段大小介于50～500bp之间。选取了200对在向日葵中得到有效扩增的引物对向日葵G101的父母本进行了多态性筛选，约25％引物具有多态性。结果表明，陆地棉EST—SSRs在向日葵中具有较高的通用性，可用于向日葵比较基因组研究和分子标记研究。

Transferability ot upland cotton EST-SSRs to upland cotton ancl sunflower were anaiyzea. 1500 pairs of upland EST-SSRs synthesized in the study were amplified in four varieties of upland cotton,their effective amplification percentage reaching 95% and their amplification products being what were expected ;each pair of primers could be used to test a certain number of bands which varied within 1 -6 and averaged at 2. 7;in every two varieties of upland cotton polymorphic bands occupied 7%-9%;400 pairs of primers chosen from the 1 500 pairs of primers were amplified in sunflower,their effective amplification percentage standing at 63% ,each pair of primers capable of testing a certain number of bands which ranged within 1-6 and averaged at 1.7,and whose sizes ranged within 50-500 bp. 200 pairs of primers that could effectively amplified in sunflower were chosen to test their polymorphisms in the parents of G101 ,finding that about 25% of them were polymorphic. The results showed that upland cotton EST-SSRs had high transferability to sunflower and thus they can be used in comparative genome and molecular marker researches.