高效液相色谱法测定聚维酮碘中的总碘含量

Determination of iodine content in povidone iodine by HPLC

目的 建立高效液相色谱法测定聚维酮碘中总碘含量的方法。方法 以Partisil SAX-10（4．6mm×250mm）柱为固定相；流动相为0．09mol／L磷酸二氢钾-甲醇（体积比9：1），用磷酸调pH3．3；流速：1．0mL／min；检测波长：223nm。结果 碘离子在3．505～17-52μg/mL范围内，峰面积与浓度呈良好的线性关系（r=0．9999，n=5）。平均回收率为99．3％，RSD=1．4％。结论 本方法简便、准确，可用于聚维酮碘的质量控制。

Objective To establish high-performance liquid chromatography for the determination of total iodine in povidone iodine. Methods The analysis was performed on Partisil SAX-10 column （4.6 mm× 250 ram）under the UV detection wavelength of 223 nm. The mobile phase was methanol-0. 09 mol/L monopotassium phosphate solution （9：1 ） （ adjusted by phosphoric acid to pH 3.3） at the flow rate of 1.0 mL/min. Results The standard curve was linear in the range of 3. 505 - 17. 525 μg/mL iodine ion （ r = 0. 9999, n = 5 ）. The average recovery was 99.3% and RSD 1. 4% （ n = 9 ）. Conclusions This method is sample and sensitive for the content determination of iodine in povidone indine.