摘要
目的 建立复方灵芝颗粒中五味子甲素含量测定的方法。方法 用高效液相色谱法测定,色谱条件为:C18柱,(5μm,250×4.6mm),流动相为甲醇-水(体积比75:25),检测波长为254nm。结果 五味子甲素在0.1608-6.432μg范围内呈现良好的线性关系(R=0.9996),平均加样回收率为99.27%,RSD=1.02%(n=6)。结论 该法操作简便、准确,重复性好,建立的定量方法可用于复方灵芝颗粒质量的控制。
Objiective To establish a method for the content determination of deoxyschizandrin in complex Lingzhi granule. Methods HPLC was performed on an ODS - C18 column (5μm,250 mm× 4.6 mm) using methanol-water (75:25 ) as the mobile phase under the detection wavelength of 254 nm, Results The linear range of deoxyschizandrin was 0, 1608 -6. 432μg, The mean recovery was 99.27% with RSD 1, 02% ( n = 6 ). Conclusion This method is simple, reproducible and accurate for the content determination of deoxyschizandrin in complex Lingzhi granule.
出处
《广东药学院学报》
CAS
2006年第1期61-62,64,共3页
Academic Journal of Guangdong College of Pharmacy