摘要
Nanog 基因在维持 ES 房间和早胚胎的房间的 pluripotency 起一个关键作用。Nanog 基因的 A 5' 胁腹顺序被报导了在 Nanog 倡导者以内是调整差别，和二个规章的元素，也就是 Oct-4 和 Sox-2 绑定地点，被识别了调整 Nanog 的 transcriptional 活动基因。在这份报告，我们识别了在鼠科的 Nanog 基因抄写的规定位于 Nanog 基因 5'-flanking 区域的二个通常认为的 Sp1 有约束力的地点的角色。变化研究证明二个地点为 Nanog 倡导者活动是必要的。胶化移动和超级移动分析证明两个地点明确地绑 Sp1 和 Sp3。而且，显著地在主导否定的 Sp1 或 Sp3 异种的表示上禁止 Nanog 倡导者活动。这些结果建议抄写因素 Sp1 和 Sp3 为鼠科的 Nanog 基因表示是重要的。
Nanog gene plays a key role in maintaining pluripotency of ES cells and early embryonic cells. A 5＇ flank sequence of the Nanog gene has been reported to be regulated differentially, and two regulatory elements within the Nanog promoter, namely Oct-4 and Sox-2 binding sites, have been identified to regulate the transcriptional activity ofNanog gene. In this report, we identified the role of two putative Spl binding sites located in the Nanog gene 5＇-flanking region in regulation ofmurine Nanog gene transcription. Mutation studies showed that the two sites were essential for the Nanog promoter activity. Gel shift and supershift analysis showed that both sites specifically bind Spl and Sp3. Furthermore, overexpression of dominant-negative Spl or Sp3 mutants significantly inhibits Nanog promoter activity. These results suggest that the transcription factor Spl and Sp3 are important for Murine Nanog gene expression.
