利用超载洗脱技术在TSK Gel CM-5PW分析柱上进行溶菌酶百毫克级制备

Preparative Separation of Lysozyme on TSK Gel CM-5PW Analyticial Column on the Scale of Several Hundred Milligrams by Overloading Elution Technique

本实验采用非线性色谱的展开方式之一——超载洗脱,在普通的分析型离子交换柱TSKGel-5PW(Φ7.5×75mm)上,一次进样蛋白质混合样150mg,成功地进行了溶菌酶的分离纯化,回收率达90%.收集的馏份经透析和冰冻干燥后,通过高效毛细管电泳(HPCE)测定纯度,得到了满意的结果,活性回收率达88%.

The separation and purification of lysozyme from 150mg protein mixture on TSK GelCM - 5PW, an analytical cation exchange column (Φ7. 5 × 75mm), by overloading elution mode of nonlinear chromatography was reported in this paper. It has been found that more than 90% of recovery and a satisfactory purity of lysozyme fraction, which was checked with standard sample by high performance capillary electrophoresis and UV spectrophotometry, was achieved.