HPLC法测定辽东楤木根皮中木皂苷C的含量

Determination of araloside C in root barks of Aralia elata(Miq.)Seem.by HPLC

目的建立HPLC法测定辽东楤木根皮中楤木皂苷C含量的方法。方法固定相为Diamonsin C18柱（250mm×4．6mm，5μm），流动相为甲醇-水-磷酸（体积比为67．0：33．0：0．2），流速为0．8mL·min^-1。检测波长为206nm，柱温为30℃。结果楤木皂苷C在20～40μg内呈良好的线性关系（r=0．9999）。平均回收率为99．7％，RSD为3．2％（n=6）。结论可用于楤木皂苷C的定量分析。

Objective To develop the analysis method for the determination of the content of araloside C in the root bark of Aralia elata （Miq.）Seem. by HPLC. Methods The chromatographic condition was Diamonsin C18 column （250 mm× 4.6 mm, 5 μm）. A mixture of methanol-water-phosphoric acid（ V： V： V = 63.0：37.0：0.2）was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was set at 206 nm at 30 ℃. Results The araloside C curve showed good linear correlation within the concentration range of 20- 40 μg （ r = 0. 999 9） ： The average recovery and RSD was 99.7 % and 3.2 96 （n = 6） respectively. Conclusions The method is convenient, reliable and with good reappearance.