摘要
目的进行流行性感冒病毒裂解疫苗的病毒灭活检测,研究病毒灭活状况。方法将单价病毒液接种鸡胚尿囊腔,第二代鸡胚尿囊液检查不应出现血凝反应。结果半成品配制前,200批单价病毒液灭活试验全部通过,单价病毒液中较高的血凝素含量会影响灭活试验的判断。结论试验表明疫苗中无活病毒存在,生产过程中血凝素含量应适当控制。
Objective To test the viral inactivation of split influenza virus vaccine. Methods Inoculate monovalent virus pools into the allantoic cavities of fertile eggs. Harvest allantoic fluid of the further passage in eggs and hemagglutination should not occur. Results All of 200 monovalent pools passed the test for inactivation before preparation of final bulk. The higher content of HA in monovalent pools could affect viral inactivation. Conclusion These results suggest that there is no live virus in the vaccine and the content of HA should be under control.
出处
《国际生物制品学杂志》
CAS
2006年第1期2-4,共3页
International Journal of Biologicals
关键词
流感疫苗
血凝素糖蛋白类
流感病毒
病毒灭活
influenza vaccine
Hemagglutinin glycoproteins,influenza virus
Viral inactivation
