茶树菇多糖的提取工艺

The Extractive Technique of Agrocybe Aegirit Polysaccharide

对茶树菇胞内和胞外多糖的提取工艺进行了研究，通过单因素法确定了胞内多糖水提法的最适条件：提取温度80～90℃，水料体积比为4：1，提取10h，共提取3次，醇析中加入3倍体积的体积分数为95％的乙醇。应用响应面分析法对胞外多糖醇析过程进行了优化。结果表明，在添加4倍体积的体积分数为95％乙醇，醇析17h，pH5．5的条件下，胞外多糖产量可达5．76mg/mL。

In this paper , we investigated the effects of various conditions of extraction on the polysaccharides of Agrocybe aegirit. The optimum extractive condition of intracellular polysaccharides was determined through single factor test as follows： extracting temperature 80℃ - 90℃ ; water： mycelia ratio 4 ： 1 ; extracting time is 10 hours; extracting in 3 times ;precipitated by addition of 3 volumes of 95 % ethanol. Optimized process of precipitated by ethanol for extracellular polysaccharides through response surface analysis. The results indicated that with addition of 4 volumes of 95 % ethanol, precipitated 17 h, pH5.5 conditions, the yield of extracellular polysaccharides was about 5.76 mg/mL.