普伐他汀对去势大鼠BMP-2表达及成骨细胞增殖的影响

Effects Of Pravastatin on BMP-2 Expression and Osteoblaster Proliferation in Ovariectomizei Rats

目的研究普伐他汀治疗去势大鼠骨质疏松症的疗效,并探讨其作用机制。方法取12周龄的雌性Wistar大鼠40只,氯氨酮(5 mg/100g)麻醉条件下,其中30只打开腹腔去除双侧卵巢,逐层缝合,另10只予以单纯打开腹腔不切除卵巢。将大鼠分为A组(模型组)、B组(治疗组)、C组(阳性对照组)、D组(假手术组)4组,饲养12周后,分别予以生理盐水(10 ml/kg)、普伐他汀水溶液(2 mg/kg)、-αD3水溶液(0.05μg/kg)及生理盐水(10 ml/kg)灌胃。灌胃12周后,处死各组动物,取右胫骨上端做病理切片,通过HE染色对成骨细胞等进行观察比较;骨形态发生蛋白-2(BMP-2)免疫组化染色观察。结果经过12周的普伐他汀水溶液灌胃治疗,明显增加成骨细胞数量(P<0.05),而且通过免疫组化染色发现有BMP-2的棕色深染,阳性细胞比例明显高于模型组(P<0.01)。结论普伐他汀可促进成骨细胞增殖、分化,其治疗骨质疏松症的机制是通过提高BMP-2的表达来实现的。

Objective To prove the experiment study was carried out to prove the efficacy of Pravastatin in ovariectomized rats and investigated its mechanism. Methods Twelve weeks affer ovariectomy, female Wistar 40 rats were divided into 4 groups： Saline （group A）, Pravastatin 2 mg/kg after ovariectomy （group B）, α-D3 0.05μg/kg （group C） and saline after sham operation （group D）. Aminals were sacrificed 12 weeks affer Pravastatin treatment. The officacy ot pravastation was exa mined aprolomaffecting osteoblast proliferation using HE stain and BMP 2 expression in bone tissue using immunohistochemistry staining. Results Compared to group A and group D, expression of BMP- 2 and the number of osteoblasters were significantly increased in group B and group C （P 〈 0.01 or P 〈0.05）. Conclusion Pravastatin robushy increaseds the counts of osteoblaster in OVX rats. High expression of BMP-2 may play an important role in Pravastatin treatment of osteoporosis.