三氧化二砷诱导子宫内膜癌细胞凋亡的研究

The molecular mechanism of the apoptosis in endometrial carcinoma cells induced by As_2O_3

目的在体外实验中,探讨三氧化二砷(As2O3)诱导子宫内膜癌细胞发生凋亡的可能性,揭示该凋亡发生与bcl-2和bax之间的关系。方法采用TUNEL染色法,定性、定量地研究As2O3与子宫内膜癌细胞凋亡的关系;通过免疫组织化学法检测凋亡相关基因bcl-2和bax的表达。结果As2O3在体外能诱导子宫内膜癌细胞发生凋亡,下调bcl-2的表达,增强bax的表达。结论诱导子宫内膜癌细胞发生凋亡是As2O3抗子宫内膜癌作用的机制之一,As2O3可能通过下调bcl-2的表达及增强bax的表达诱导子宫内膜癌细胞发生凋亡。

Objective To investigate the apoptosis in endometrial carcinoma cells induced by As2O3, and the expression of bcl-2 and bax. Methods In vitro experiments, TUNEL staining method was used to quantitatively and qualitively detect the apoptosis status of endometrial carcinoma cells HEC-1A before and after the As2O3 treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene bcl-2 and bax. Results As2O3 was able to induce the apoptosis in endometrial carcinoma cells. As2O3 can reduce the expression of apoptosis-regulated gene bcl-2, and up-regulate the expression of apoptosis-regulated gene bax. Conclusions As2O3 was able to induce the apoptosis in endometrial carcinoma cells. This apoptosis may be mediated by down-regulation of apoptosis-regulated gene bcl-2 and up-regulation of bax.