利用农杆菌介导法将柠檬酸合成酶基因(CS)导入籼稻品种明恢86

Introduction of Citrate Synthase Gene (CS) into an Elite Indica Rice Restorer Line Minghui 86 by Agrobacterium -mediated Method

磷是生命的必需元素之一,在作物的生长发育中起着重要的作用。然而,大多数土壤中有效磷的含量很低,而作为磷肥生产的磷矿资源正趋于耗竭,与此同时,土壤中的磷大部分以作物难以利用的形态存在。已有的研究表明植物通过分泌柠檬酸活化土壤中难溶性无机磷从而提高了土壤磷的可利用性。本研究采用根癌农杆菌介导法将柠檬酸合成酶(citratesynthase)基因CS导入杂交籼稻优良恢复系明恢86,共获得48株T0再生植株。经PCR检测,其中22株为转基因阳性植株。对阳性转基因植株的Southern及Northern分析表明,外源基因已整合到了水稻基因组中并得以有效表达。转基因植株后代的生理学和农艺学性状的研究正在进行之中。

Phosphorus （P） is a necessary macronutrient for plant growth and development. However, low available phosphate is the most common characterization of soils and most of phosphorus in soil is unavailable to plants, on the other hand, the exploitable phosphorus resources in nature tends to be used up now. A few studies that plant secreted citrate acid from roots to activate the unavailable inorganic phosphorus into the available form have been done. In this study the citrate synthase gene （CS） was introduced into an elite indica restorer Minghui 86 by Agrobacterium-mediated method. Twenty-two transgenic plants were identified by PCR detection from initial 48 To regenerated plants. Southern blotting and Northern blotting assay indicated that the foreign gene has been successfully integrated into the genome of the recipient dee variety and effectively expressed in cells. Further investigations on physiological and agronomic traits of T2 homozygous lines are undergoing.