卵巢移植技术应用于胰腺癌转基因小鼠模型繁殖传代研究

Application of Ovary Transplantation in Pancreatic Cancer Transgenic Mice Propagation

目的研究卵巢移植在胰腺癌转基因小鼠模型保种传代中的应用。方法通过显微手术将SV40T阳性转基因小鼠的卵巢原位移植给相同背景品系去除卵巢的正常FVB雌鼠体内。移植后与正常FVB雄鼠配种,后代以PCR检测确定阳性,统计后代发病情况。并对移植受体鼠进行卵巢的组织切片观察。结果实验中移植用供体鼠19只。成功移植给32只受体。其中有21只移植受体怀孕产子,得到后代159只,存活96只,PCR检测阳性34只,阳性率约为25.4%。结论卵巢移植可被用于转基因小鼠的传代繁殖保种。

Objective To study the application of ovary transplantation for preserving pancreatic cancer transgenic mice. Methods Ovary of SV40T positive transgenic mice were transplanted into ovarectomized FVB female mice through microsurgery,then were crossed with normal FVB male. The positive offsprings were confirmed by PCR. Ovary tissue slides of foster mother were examined. Results Ovaries of 19 donors were transplanted into 32 female mice, among which 21 were pregnant. 96 in 159 offsprings survived. 35 with a positive transgenic mice were identified by PCR, with a positive rate of 25.4%. Conclusion Ovary transplantation may be used for transgenic mice preservation.