微卫星座位对实验动物beagle犬的遗传分析

Genetic Analysis of Laboratory beagle Dogs by Microsatellite Loci

目的对美国进口、广州自养beagle犬基因组中存在的微卫星结构进行分析,研究其群体的微卫星多态性,以此探索在分子水平上对作为实验动物的beagle犬进行检测。方法通过微卫星分子标记技术进行遗传背景分析,并结合微卫星位点测序结果,研究DNA分子特征。结果在研究位点上共发现6个复等位基因,进口犬群体共有6个等位基因片段,自养犬群体共有5个等位基因片段,根据基因型计算各群体等位基因频率,由相关公式计算杂合度、群体多态信息含量(PIC)、基因纯合率、基因分化系数。结论两群体的杂合度、PIC值均较高(分别为0.7010、0.6747和0.7876、0.7515),基因分化系数很低(0.021),表明两群体没有形成明显的独立群。

Objective In order to assess and verify the identity of beagle dogs bred in Guangzhou and from USA on molecular level, polymorphism of microsatellite loci of the beagle dog genome were analysed. Methods Using the short tandem repeats （STR） technique and sequencing of microsatellite loci to assess the DNA of the two dog populations. Results Six alleles were found in the samples. Among them, the populations of dogs from USA and dogs bred in Guangzhou had 6 and 5 alleles, respectively. Rates of alleles of microsatellite loci were calculated according to genotypes. Heterozygosity, polymorphism information content （ PIC）, rate of homozygosity, coefficient of gene differentiation of microsatellite loci were calculated by means of correlated formulas. It showed that individuals bred in Guangzhou had the heterozygosity 0.7010 and PIC 0.6747, In comparison, the individuals from USA had the heterozygosity 0.7876 and PIC 0.7515. Coefficient of gene differentiation is small （0.021）. Conclusion Our findings indicate that the two populations did not form different independent groups.