消炎痛对白色念珠菌菌丝形成的影响

The effect of the Indomethacin on the hypha formation of candida albicans

目的:探讨环氧化酶抑制剂消炎痛对白色念珠菌形态发育的影响。方法:分别用含不同浓度消炎痛的RPM I1 640加10%小牛血清的液体培养基和玉米-吐温80固体培养基培养3株白色念珠菌,观察白色念珠菌形态发育变化。结果:消炎痛对白色念珠菌芽管和菌丝形成的抑制作用与其剂量相关。在液体培养基中,消炎痛浓度为0.75 mM时可抑制50%芽管形成,浓度为2.0mM时可完全抑制芽管和菌丝形成;而在固体培养基中,消炎痛浓度为1.0mM时可抑制菌丝和厚壁孢子的形成,浓度为2.0 mM时,几乎没有菌丝形成。结论:消炎痛可以抑制白色念珠菌芽管、菌丝的形成,阴道内给予消炎痛将有助于治疗念珠菌性阴道炎。

Objective： To investigate the effect of the cyclooxygenase inhibitors - Indomethacin on the morphogenesm ot Ganalda Albicans. Methods： Three strains of Candida Albicans were grown in RPMI 1640 ＋ 10% new fetal serum liquid medium and corn - tween 80 solid medium, which contained different concentration of Indomethacin, to observe the changes of them in morphogenesis. Results： Indomethacin inhibited the formation of germ tube and hypha of Candida Albicans by dose dependence. In the liquid medium, lndomethacin could inhibit 50%germ tube fomation at 0. 75mM level, while it could fully inhibit germ tube formation at 2.0mM level; In the solid medium, Indomethacin could inhibit the formation of hypha and chlamydospores at 1.0mM level, and no hypha formed at 2. 0mM level. Conclusion： Indomethacin can inhibit the formation of germ tube and hypha of Candida Albicans. The administration of Indomethacin should be beneficial in treatment of vulvoviginal candidiasis.