整合素α_5β_1在卵巢上皮性癌中的定性及定量研究

The analysis of the expression of integrin α_5β_1 in ovarian epithelial neoplasms

目的:检测整合素α5β1在卵巢上皮性肿瘤中的蛋白表达,探讨其与卵巢癌淋巴结侵袭、转移的关系。方法:应用免疫组化法,检测100例卵巢上皮性肿瘤(其中上皮性癌54例、交界性肿瘤26例、良性肿瘤20例)。石蜡包埋标本中整合素α5β1的蛋白表达,运用计算机图像自动分析系统进行定量分析。结果:①定性表达:整合素α5β1在淋巴结转移组和未转移组中的阳性表达率为25.00%和61.11%(P<0.05);在不同病理分级中的阳性表达率依次为53.85%、58.82%和12.50%,1、2级和3级间比较,差异具有显著性;在良性、交界性及恶性肿瘤标本中的阳性表达率分别为80.00%,75.00%和37.04%,前2者与后者间比较,具有显著性统计学差异。②定量结果:整合素α5β1表达的阳性细胞面积,按良性、交界性和恶性肿瘤的顺序呈递减趋势,其中,良性与恶性肿瘤3级的阳性细胞面积为11 576.479 5和1 960.917 83,(P<0.05);良性与恶性肿瘤3级的灰度值比较有显著性差异。结论:整合素α5β1在卵巢上皮性癌的侵袭和转移过程中起重要作用,其低表达和失表达提示肿瘤恶性程度高,预后不良。

Objective： Through the investigating the molecular expressions of integrin α5β1, in ovarian epithelial neoplasms, this study is trying to establish the relationship between the lymphatic spread of the tumor with these molecules. The study may shed some lights on the scientific basis to make the diagnosis of the lymphatic spread of the tumor as well as provide valuable information to predict the prognosis of the tumor. Methods： 100 cases were examined through Elivision TM, an immune histochemistry method, for the expression of integrin asti, in ovarian epithelial neoplasms. The system of image analysis was used to measure the expression of various molecules quantitatively. The results were also tested using SAS6. 12 and the SPSS 10. 0 software packages. Results： 1. Qualitative and semi -quantitative results： The expression levels of integrin α5β1 in the ovarian epithelial neoplasms have downward tendency in the order of benign, boundary and ma- lignant neoplasms. There is significant difference on the expression levels of integrin α5β1 between the group with metastases and the group without metastases. 2. Quantitative examination result ： The total positive cell area of the integrin α5β1 expression in the tumor has downward tendency in the order of benign, boundary and malignant cases, and the gray degree has upward tendency. Conclusion： The molecular expressions of integrin α5β1in ovarian epithelial neoplasms are related to biological behavior of the tumor. Decreasing or lack of the expression of integrin α5β1, suggest the rapid development, high degree of malignancy and poor prognosis; The histological classification and pathological degrees of the ovarian epithelial neoplasms is still basis for diagnosing the lymphatic spread and determining the prognosis of the tumor.