大中药复方的色谱指纹图谱应用研究

Applied study of large TCM compound recipe fingerprint

目的:建立澳洲方的指纹图谱,为较大复方化学研究提供整体性方法。方法:通过澳洲方及其药材LC/UV/MS指纹图谱的建立,将药材与复方指纹峰的进行比较,抽提出复方指纹特性,根据测试结果,将指纹峰对应的保留值,在线紫外图谱以及MS图谱收集组成澳洲方指纹图谱库。结果:区分了药材归属的指纹峰,多药材来源的指纹峰和无归属峰三个特征类别层次,用于对复方样品的识别,10批样品的结果发现,利用特征数据组成的指纹图谱识别准确率高于单纯使用LC/UV或者LC/MS计算相似度的结果,其中按照中位值比较,上述特征数据1 0批的偏差范围±2.7%,而LC/UV为±10.5%,LC/MS为±11.2%。结论:抽提复方中具有药材归属的指纹信息组成指纹图谱库,利用其中特征数据的比较,可以准确灵敏地反映出复方的质量特性和物质特征,在对复方质量评价的全面性以及复方药材组合关系的分析上有着良好的识别和鉴别功能,可为大复方物质基础研究提供途径。

AIM： Provided the integrated method for large TCM recipe chemical study with the establishment of Aozhou formulae, imcluding 20 herbs, METHODS ： With the comparison of Aozhou formulae and its herbs fingerprint chromatogram, it extracted fingerprint characters from chromatogram after the method establishment. According to the test results, the retention data, on -line UV and MS spectral were collected as the Aozhou formulae fin- gerprint database. Combined with three character classified data layers named as herb jurisdic;tion, multi herb jurisdiction and no herb jurisdiction, the Aozhou formulae was identified by database mentioned above with the 10 batches results. RESULTS： It presented that formulae identification by character data was more accurate than by LC/ UV or LC/MS data merely. According to the comparison with median, the deviation were ±2.7%, ±10.5%, ±11.2% identified by character, LC/UV and LC/MS respectively. CONCLUSION： From the method described in this article, it could be more accurate and sensitive to reflect quality and substance characteristic by the comparison of characters extracted from compound recipe fingerpnnt chromatogram. And also it provided method to study the substance basement of large TCM formulae,