均匀设计和回归分析优选莪术超临界萃取工艺

Study on the supercritical CO_2 extraction of curcumolin Curcuma with uniform design and regression analysis

目的:以莪术醇含量为响应指标,优化莪术有效成分的超临界CO2萃取工艺。方法:通过均匀试验设计,采用气相色谱法测定莪术醇的含量,采用逐步非线性回归、通径分析和等高线图优化提取工艺。结果:莪术的超临界CO2最佳萃取工艺为萃取温度40℃、萃取压力20 MPa、动态萃取时间120 m in、改性剂用量24 mL、改性剂浓度70%、静态萃取时间20 m in;模型预测值的绝对误差小于2%。结论:回归模型极为显著,并且具有很高的预测精度,可以很好对试验结果进行预测。

AIM： To optimize the extraction process for the active components in Curcuma by supercritieal CO2 as guided by the contents of eureumol in Curcuma. METHODS： Gas chromatography was used to determine the contents of cureumol in the extract. Uniform design and path analysis were used to optimize SFE process. RESULTS： The optimum process was established as following：20 MPa as extracting pressure, 40℃ as extracting temperature, 120 minutes as dynamic extracting time and 20 minutes as static extracting time, 24 mL of 70% alcohol as modifier. The absolute error of tile predicted value from the model was smaller than 0.6%. CONCLUSION： The regression model is very notable and reasonable and can forecast results precisely.