正交设计在灯盏花组织培养中的应用

Application of Orthogonal Design in Tissur Culture of Erigerm breviscapus(Vant.)Hand.-Mazz

针对灯盏花自然繁殖率低下的情况,探讨了利用植物组织培养方法解决灯盏花生产中繁殖的问题。按正交实验设计的原则,摸索出建立灯盏花组织培养技术体系的最佳条件:不定芽诱导的培养基是MS+6-BA 1.0mg/L+NAA 0.1 mg/L,生根培养基是MS+NAA 0.5 mg/L+IAA 0.2 mg/L。

Because the fruiting rate and germinative percentage of Erigerrn breviscapus （Vant.）Hand.- Mazz are lower, tissue culture was explored to solve the propagating problem in the production. By using the orthogonal experiment design, the experimental result shows that the best culture medium of inducing adventitious bud was MS ＋ 6 - BA 1.0 mg/L ＋ NAA 0.1 mg/L, the best culture medium of root was NAA 0.5 mg/L ＋ IAA 0.2 mg/L