摘要
主要针对枣组织中多糖严重干扰基因组DNA提取质量的问题,比较了常规CTAB法、TE3D法和改良CTAB法3种方法的处理效果。结果表明:常规CTAB法提取DNA难以除去多糖类杂质;TE3D法提取的DNA多糖杂质少,但产率低,易降解,褐化严重;改良CTAB法提取DNA产率高,无明显降解,杂质少,D260nm/D280nm比值1.80左右,通过基因组DNA-AFLP指纹图谱分析,完全满足实验要求。并对改良CTAB法的关键步骤作了具体分析讨论。
In order to solve the problem of polyhexose interfering the genomic DNA extraction of Zizyphus jujuba Mill. , 3 methods, the conventional CTAB, TE3D and improved CTAB were compared. The results showed that the conventional CTAB method was difficult to get rid of polyhexose completely ; the TE3D method could remove polyhexose effectively, but extraction rate was very low, DNA browned and degraded seriously; the improved CTAB method was an effective way to solve all kinds of problem, DNA concentration was up to 1000 ng/μL, degradation was light and impurity was little, A260/ A280 was 1.80 or so. When tested through the DNA-AFLP fingerprinting, they could meet experiment requirement perfectly. In addition, the key steps and main features of the improved CTAB method are discussed.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第2期310-312,共3页
Journal of Fruit Science
基金
国家科技基础条件平台工作重点项目(2003DKA30390-23)
农业部作物种质资源保护项目(NB05-070401-2)。
关键词
枣树
DNA提取
改良CTAB法
Zizyphus jujuba Mill.
DNA extraction
Improved CTAB method
