粘质沙雷氏菌酶促过氧化氢降解对氨基苯酚的研究

DEGRADATION OF 4-AMINOPHENOL BY HYDROGEN PEROXIDE OXIDATION USING ENZYME FROM SERRATIA MARCESCENS AS CATALYST

利用源于粘质沙雷氏菌(Serratia marcescens)AB 90027的酶催化过氧化氢氧化降解对氨基苯酚, 对降解过程的影响因素和对氨基苯酚的降解途径进行了研究,并分析了酶在细胞中的存在位置．结果表明, 降解500mg·l-1对氨基苯酚溶液50ml,其适宜的条件为:H2O23ml,温度40℃-60℃,pH 9．0-10．0;在酶的催化作用下,H2O2氧化对氨基苯酚首先生成对苯醌,进一步氧化生成顺丁烯二酸、反丁烯二酸、草酸等有机酸并最终转化为CO2和H2O．可催化降解对氨基苯酚的酶为胞外酶．

This paper reports on the degradation of 4-aminophenol using hydrogen peroxide as oxidizer and enzyme from Serratia marcescens AB 90027 as catalyst. The effecting factors during degradaiton were studied and the degrading mechanism was clarified. Also the situation in the cell for the enzyme which could catalyze to degrade 4-aminophenol was researched. The results showed that to degrade 50ml 4-aminophenol whose concertration was 500mg·1^-1, the optimal conditions were： the volume of H2O2was 3ml , the temperature was 40℃-60℃ and the pH was 9-10. In the degradation process, first 4-aminophenol converted to benzoquinone and gave out NH3, then orgaic acids including maleic acid, fumaleic acid, and oxalic acid were formed, at last CO2and H2O was generated as final products. The enzyme which could catalyze to degrade 4-aminophenol was mainly extracellular enzyme.