猪血中血红素提取工艺研究

Study on the reaction condition of the heme extraction of the porcine blood

对猪血中血红素的提取工艺进行了研究,通过正交实验确定了提取血红素的最优工艺条件,即:溶血时,添加去离子水3倍,氯仿4倍;分离血红素是,加丙酮6倍、同时调节pH值为3;结晶血红素加1mol/L醋酸钠1倍,调节pH值为5。根据本工艺可从1000ml猪血红细胞中提取4.9201g、纯度达60%的血红素。

extracting heine form pig blood was studied in this paper. It was used orthogonal experiment to investigate the best conditions of extraction techniques for heine, that is： dissolve the blood with three times clean water, four times chloroform; then add six times acetone in it when setting it apart, and regulate the pH to 3; and a time 1 mol/L sodium acetate in when heine crystallizing, regulate the pH to 5.according to this, it can be accquined about 4.9201g heine with purity at 60% form 1000ml pig blood.