膳食诱导肥胖抵抗大鼠下丘脑NPY mRNA及蛋白的表达

Study on the Neuropeptide Y mRNA and Protein Expression of Diet-induced Obesity Rats

目的研究膳食诱导肥胖(DIO)及肥胖抵抗(DIO-R)大鼠下丘脑弓状核神经肽Y mRNA及蛋白表达情况,探讨神经肽Y与肥胖抵抗的关系。方法高脂饮食建立DIO与DIO-R大鼠模型,分别于2周、12周末处死,采用原位杂交和免疫组化方法检测下丘脑弓状核神经肽Y的mRNA和蛋白表达情况。结果实验2周末,DIO大鼠神经肽Y mRNA和蛋白阳性面积分别为(0.563 8±0.100 5),(0.610 9±0.143 4)mm2,高于DIO-R(0.411 5±0.085 1),(0.523 1±0.096 3)mm2和对照组大鼠(0.400 4±0.062 2),(0.445 3±0.157 8)mm2;神经肽Y mRNA和蛋白积分光密度分别为9 025.48±2 192.32,7 786.39±1 423.02,高于DIO-R(7 651.79±2 279.21,5 447.16±3 207.09)和对照组大鼠(6 499.24±2 342.94,5 530.83±841.13)。结论神经肽Y mRNA和蛋白表达降低且保持敏感性可能与肥胖抵抗有关。

Objective To investigate NPY mRNA and protein expression in the ascuate nucleus of hypothalamus of diet-induced obesity（DIO） and diet-induced obesity resistant（DIO-R） rats, and to explore the relationship between NPY and obesity-resistant. Methods The DIO and DIO-R rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats. Results After 2 weeks of diet, the areas of NPY mRNA and protein expression of DIO rats were（0.563 8 ± 0. 100 5 ） mm^2 and（0.610 9 ± 0.143 4）mm^2, higher than those of DIO-R（0.411 5 ± 0.085 1,0.523 1 ± 0.096 3） and the control rats（0.400 4 ± 0.062 2,0.445 3 ± 0.157 8 ）. The IOD of NPY mRNA and protein expression of DIO were. 9 025.48 ± 2 192.32 and 7 786.39 ± 1 423.02, higher than DIO - R （ 7 651. 79 ± 2 279.21,5 447.16 ± 3 207.09） and the control rats （6 499.24 ± 2 342.94,5 530.83 ± 841.13）. Canclusion Decreased NPY mRNA and the kept NPY sensitivity might be related to obesity-resistant.