自来水痕量有机污染物检测及致突变性研究

Study on organic pollutants of tap water and its genotoxicity in Kunming

目的:在对自来水中痕量有机污染物检测的基础上研究其对人体健康的潜在危害。方法:采集某市3个自来水厂出厂水(A、B、C)和对照水样(D),XAD-2树脂富集自来水样中的痕量有机物,气相色谱/质谱(GC/MS)联用法检测分析;鼠伤寒沙门氏菌回复突变试验(Am es试验)检测其致突变性。结果:在选定的条件下,本次试验累计检出痕量有机物335种,其中EPA(美国环保署)优先污染物10余种,致癌、致畸、致突变20余种。A水厂出厂水在第一次Ames试验中TA98和TA100在不加S9时呈阳性(MR>2);A、B、C水厂出厂水在第二次试验中呈可疑阳性(±),存在剂量反应关系。对照水样Ames试验结果为阴性。结论:GC/MS联用分析、Ames试验联合运用是研究水中有机污染物致突变性的有效方法。

Objective：To study the main organic pollutants in tap water and its genotoxicity in Kunming city. The tap water quality and its genotoxicity was evaluated. Methods： A volume of 150 liter tap water sample from three waterworks （A, B, C） and a source water （D） were collected. The organic pollutants were extracted by XAD - 2 resin, then washed by methanol and acetone. The organic extractions were detected by using Gas Chromatography/Mass Spectrometry （GC/MS）. In vitro Salmonella assay （ Ames test） was used to detect the genotoxicity effect of organic pollutants. Results：All the 335 organic pollutants were monitored under certain condition in the above - mentioned water samples, including up to 10 kinds of EPA Priory pollutants and up to 20 kinds of mutagens and carcinogens. The first Ames test results of organic extraction from waterworks A was positive （ MR 〉 2） and the second was suspectedly positive and showed dose response relationship. The first Ames test results of organic extraction from waterworks B and C were both suspectedly positive and the second were suspectedly positive and showed dose response relationship. The outcome of control sample was negative in the Ames testes. Conclusion ：The combination of GC/MS and Ames test is a rapid and effective method for evaluating organic pollutants in tap water.