固相时间分辨荧光免疫分析螯合剂(Ⅰ)中间体—4,4′-二溴-6,6′-二甲基2,2′-联吡啶的合成

Synthesis of media of chelate solid-phase time-resolved fluorescence immunoassay—synthesis of 4,4′-dibromo-6,6′-dimethyl-2,2′-bipyridine(Ⅰ)

目的:合成固相时间分辨荧光免疫分析螯合剂的中间体-4,4′-二溴-6,6′二甲基2,2′-二吡啶。方法:根据自行设计的合成路线,以2-氨基6-甲基吡啶为原料,经重氮化、乌尔曼偶联合成6,6′-二甲基-2,2′-二吡啶,再经氧化、硝化、溴化、脱氧等合成4,4′-二溴-6,6′二甲基2,2′-二吡啶。结果:经元素分析、红外光谱,核磁共振波谱等方法证明:成功的合成了4,4′-二溴-6,6′二甲基2,2′-二吡啶。结论:通过自行设计路线完成了4,4′-二溴-6,6′二甲基2,2′-二吡啶的合成,为固相时间分辨荧光免疫分析的应用提供了有价值的中间体和制备方法。

Objective：To synthesize 4,4＇ - dibromo - 6,6＇ - dimethyl - 2,2＇ - bipyridine that is media of chelate solid - phase time - resolved fluorescence immunoassay. Methods： 6,6＇ - dimethyl - 2,2＇ - bipyridine was synthesized by diazotization and Ullmann＇s couple of 2 - amino -6 - methylpyridine on the basis of self - design way. 4,4＇ - dibromo -6,6＇ - dimethyl -2,2＇ -bipyridine was synthesized by oxidation, nitrition and deoxidation of 6,6＇ -dimethyl -2,2＇ -bipyridine. Results ：4,4＇ -dibro- mo- 6,6＇ - dimethyl -2,2＇ -bipyridine was synthesized successfully and confirmed by the methods of element analysis, IR and ^1HMR. Conclusion： The valuable media and preparation method were offered in application of the method of solid - phase time - resolved fluorescence immunoassay. The 4,4＇ - dibromo - 6,6＇ - dimethyl - 2,2＇ - bipyridine is synthesized completely by self - designed way.