3种霍乱弧菌检测方法在外环境标本中的应用研究

Application of three Vibrio cholera′s detection methods to detection of infectious water samples

目的:通过改进霍乱弧菌检测的技术手段,提高外环境标本中霍乱弧菌的检出率。方法:将常规分离鉴定法、PCR检测法和胶体金免疫层析试验法同时用于检测霍乱流行期间的外环境标本,并对其现场应用结果进行比较分析。结果:3种方法检测80份外环境标本霍乱弧菌的结果显示:它们均未出现假阳性,3种方法各有优势,且结果相互符合性好,但也存在各自弱点。结论:只用单一种方法来检测外环境标本霍乱弧菌存在明显不足。建议在外环境霍乱监测中选用胶体金法进行初筛,再用常规法和PCR法检测,最大限度减少漏检的可能性。

Objective：To improve the detection Vibrio cholera in infectious water and food samples positive rate by improving the Vibrio cholera＇s detection methods. Methods： Vibrio cholera was detected in infectious water and food samples by using routine separation method, PCR and gold -immunochromatography assay （GICA） , and the results had been compared with each other. Results：Results of detecting Vibrio cholera in 80 infectious water samples showed that they all didn＇t appear false positive. Each of them has its advantage and weakness. Conclusion：Using one method to detect Vibrio cholera in infectious water has evident deficiency. It is suggested to use GICA to screen first, then use routine method and PCR to detect so as to decrease the possibility of false determination.