结香树的组织培养及植株再生技术研究

A Study on in Vitro Plantlet Regeneration of Edgeworthia chrysantha

以结香树幼嫩茎段为外植体研究其组织培养和植株再生技术.结果表明:结香树茎段组织培养的基本培养基为WPM,幼嫩茎段诱导芽的适宜培养基为WPM+IBA 0.2 mg.L-1+6-BA 3 mg.L-1,幼芽形成根的适宜培养基为1/2 MS+IBA 0.5mg.L-1,该研究结果为结香树的快速繁殖及工厂化生产奠定了基础.

This paper deals with in vitro plantlet regeneration of Edgeworthia chrysantha L. with young stem segments as an explant. Results show that the basal medium of the shoot induction was WPM , and that the appropriate media for the shoot induction from young stem segments and the rooting from shooting were WPM ＋ IBA 0.2 mg·L^-1 ＋ 6-BA 3 mg·L^-1 and 1/2 MS ＋ IBA 0.5 mg·L^-1 respectively. The research results provide references to the quick propagation and factory production of E. chrysantha.