珍稀竹种巨龙竹组织培养研究

Study on Tissue Culture of Dendrocalamus sinicus

对巨龙竹幼年竹和成年竹材料采集、处理、试管引入、丛芽诱导、丛芽增殖和生根、再生植株炼苗等进行系统研究,采用正交设计筛选出各阶段的最佳培养基。结果表明:丛芽增殖倍数与BA用量成正比,但随着BA用量加大或长期在高浓度BA上培养易产生花芽,说明BA有使丛芽细胞从营养状态向生殖状态转变的作用;KT在丛芽高生长方面起主导作用,能使丛芽节间伸长,使细胞从生殖状态向营养状态转变;椰乳有改善丛芽或苗生长的作用。在诱导丛芽生根过程中,IBA起主导作用,配合少量NAA使用可得到生根良好、移栽成活率较高的再生小植株。巨龙竹成年竹组织培养与外植体采集部位、采集时间、采集地等相关。

Tissue culture of young and mature bamboo of Dendrocalamus sinicus was studied systematically from aspects of explant collection, disposal, in vitro; and shoot inducement, proliferation, rooting; and plantlet hardening and so on in this experiment. The best media for every stage were discussed by orthogonal experiment. The results showed that the dosage of hormone BA and the shoot multiplication were proportioned positively. But if the dosage of BA enlarged or a long time＇s culture made in high thick of BA, flower buds would be seen. So BA had the function of making cells become progenitive performance from nutritious performance. Hormone KT was the main factor in height growth of shoot multiplication, which could make nodes elongate, make buds grow well and make cells become nutritious performance from progenitive performance. Coconut water （CW） had function of enhancing growth. Hormone IBA was the main factor in rooting. A few hormone NAA in combination with IBA could make rooting well and growing well. And the plantlets showed highly survival rate in hardening. The successes of explants in vitro and induced multiplication of shoots were related to the collected time and collected section etc. of the explants.