摘要
目的初步建立异质性甲氧西林耐药金黄色葡萄球菌(heterogeneous m ethec illin-resistantStaphylococcus aureus,hetero-MRSA)检测方法,并对hetero-MRSA检测的临床意义进行探讨。方法采用菌谱分析法和K-B法,对60株经V itek 32检测为甲氧西林敏感金黄色葡萄球菌(m ethec illin susceptib leStaphylococcus aureus,MSSA)进行hetero-MRSA检测。结果菌谱分析法检出12株hetero-MRSA,K-B法检出2株hetero-MRSA。结论菌谱分析法对hetero-MRSA的检出率(20%)显著高于K-B法(3.3%)。MSSA对甲氧西林的异质性耐药不容忽视。
Objective To develop a new method for the isolation of heterogeneous methecillin-resistant staphylococcus aureus ( hetero- MRSA) and study its application in clinical practice. Methods Both the methods of disc agar diffusion and population analysis were used to detect hetero-MRSA from 60 strains of methecillin susceptible staphylococcus aureus(MSSA) which were identified by VITEK AMS. Results Twelve subclones of hetero-MRSA were isolated by population analysis ,while only 2 subclones were identified by disc agar diffusion. Conclusion Population analysis is an effective method for the isolation of hetero-MRSA, and its detection rate is higher than that of disc agar diffusion. The heterogeneous methecillin resistance of MSSA should not be overlooked by medical workers.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2006年第2期94-96,共3页
Chinese Journal of Clinical Laboratory Science
基金
苏州市科教兴卫青年专项基金(SWQ15)