菌谱分析法用于异质性甲氧西林耐药金黄色葡萄球菌检测

Population analysis as a useful method for isolation of herogeneous methecillin-resistant staphylococcus aureus

目的初步建立异质性甲氧西林耐药金黄色葡萄球菌(heterogeneous m ethec illin-resistantStaphylococcus aureus,hetero-MRSA)检测方法,并对hetero-MRSA检测的临床意义进行探讨。方法采用菌谱分析法和K-B法,对60株经V itek 32检测为甲氧西林敏感金黄色葡萄球菌(m ethec illin susceptib leStaphylococcus aureus,MSSA)进行hetero-MRSA检测。结果菌谱分析法检出12株hetero-MRSA,K-B法检出2株hetero-MRSA。结论菌谱分析法对hetero-MRSA的检出率(20%)显著高于K-B法(3.3%)。MSSA对甲氧西林的异质性耐药不容忽视。

Objective To develop a new method for the isolation of heterogeneous methecillin-resistant staphylococcus aureus （ hetero- MRSA） and study its application in clinical practice. Methods Both the methods of disc agar diffusion and population analysis were used to detect hetero-MRSA from 60 strains of methecillin susceptible staphylococcus aureus（MSSA） which were identified by VITEK AMS. Results Twelve subclones of hetero-MRSA were isolated by population analysis ,while only 2 subclones were identified by disc agar diffusion. Conclusion Population analysis is an effective method for the isolation of hetero-MRSA, and its detection rate is higher than that of disc agar diffusion. The heterogeneous methecillin resistance of MSSA should not be overlooked by medical workers.