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金黄色葡萄球菌万古霉素体外诱导中介株凝固酶基因序列改变

Studies on relationship between resistance to vancomycin and changes of coagulase gene sequences of S.aureus
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摘要 目的研究万古霉素敏感金黄色葡萄球菌经过体外诱导变成万古霉素中介耐药的金黄色葡萄球菌后凝固酶基因序列有无改变。方法利用酶切及SSCP技术检测凝固酶基因序列改变情况,并测序进行确证。结果检测到1株金黄色葡萄球菌凝固酶基因序列有改变。结论凝固酶基因酶切位点的改变及其他位点碱基的改变,可能是凝固酶活性降低的原因之一。 Objective To induce vaneomyein-resistantant S. aureus in vitro and to observe the changes of coagulase gene sequences of S. aureus and its relationship with vancomycin resistance. Methods DNA sequences of the coagulase were examined by polymerase chain reaction-sigle strand conformation polymorphism （PCR-SSCP） method and were identified by sequencing. Results The altered DNA se- quence of coagulase in 1 strain of S. aureus was found. Conclusion The variation of DNA sequences of coagulase gene is probably linked to vancomycin-resistance in S. aureus.
作者 代洪 范学工
机构地区 中南大学湘雅医院传染科
出处 《临床检验杂志》 CAS CSCD 北大核心 2006年第2期97-99,共3页 Chinese Journal of Clinical Laboratory Science
关键词 万古霉素 耐药 凝固酶 基因序列 vancomycin drug resistibility coagulase gene sequences
分类号 R446.5 [医药卫生—诊断学]
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参考文献13

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临床检验杂志
2006年 第2期

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