Eotaxin-3基因多态性与变应性哮喘相关研究

Association between polymorphisms of eotaxin-3+2497T/G and asthma

目的研究湖北地区汉族成人+2497T/G单核苷酸多态性与哮喘易感性、嗜酸性粒细胞计数以及血浆总IgE水平相关性。方法用聚合酶链反应-单链构象多态性-4引物聚合酶链反应的方法进行分析。结果eotaxin-3+2497位G等位基因的频率在哮喘组与对照组、哮喘组中TT、TG基因型的IgE浓度及EOS数量差异有显著性,P值分别为0.011,0.021和0.029。结论eotaxin-3+2497T/G多态性与哮喘易感性、EOS数量及IgE水平相关。

Objective To study the single nucleotide polymorphisms of ＋ 2497 in eotaxin-3 and its association with asthma susceptibility, total IgE level and eosinophil count in adult population Of the Han nationality from Hubei province of China. Methods Polymerase chain reaction-single strand conformation polymorphism and tetra-primer polymerase chain reaction were used to identify the single nucleotide polymorphisms. Results The frequencies of eotaxin-3 ＋ 2497 G allele between asthma group and control group were significantly different （P value ：0.011 ）. The total levels of plasma IgE and peripheral eosinophil counts between TT and TG genotypes in asthma group were significantly different and the P values were 0. 021 and 0. 029 respectively. Conclusion Polymorphism of eotaxin-3 ＋ 2497 is associated with the susceptibility of asthma,peripheral eosinophil counts and total levels of plasma IgE.