麝香壮骨膏中樟脑和水杨酸甲酯含量的测定

Determining the Content of Camphor and Wintergreen oil in Musk Strong Bone Paste

建立麝香壮骨膏中樟脑和水杨酸甲酯含量的测定方法,并测定麝香壮骨膏中两种主要有效成分的含量,以控制麝香壮骨膏生产的质量。采用GC法测定麝香壮骨膏中樟脑和水杨酸甲酯的含量;色谱条件为:毛细管柱PEG-20M,30m×0.32mm×0.25μm,柱温:165℃,进行口温度:190℃,检测器温度:200℃;N2:50Kpa,H2:50Kpa,A ir:50Kpa;进样量:1μl,毛细管柱采用分流进样方式,分流比1:10。在选定的色谱条件下,樟脑和水杨酸甲酯在0.7785-31.14μg和0.7537-15.07mg范围内与峰面积呈良好的线性关系,平均回收率分别为98.35%和102.72%,RSD分别为0.6%和1.20%;本实验为麝香壮骨膏的质量分析提供了一种准确可靠的检测方法。

This paper studies the determination method of the camphor and the wintergreen oil content in musk strong bone paste and determines the content of the two major components in musk strong bone paste so as to control the quality of the musk strong bone paste. Use Gas Phase Chromatogram Approach to determine the content of camnpbor and the wintergreen oil in musk strong bone paste; The chromatogram condition is： Size of the Capillary vessel column： PEG -20M, 30m × 0.32mm × 0. 25μm, temperature of the capillary vessel column： 165℃, temperature of the Entrance of the capillary vessel column：190℃, temperature of the detector： 200℃;N2： 50Kpa, H2： 50Kpa, Air： 50Kpa; Sample quantity： 1μl. The capillary vessel column uses the diffluence sample flow mode, the diffluence ratio is ： 1 ： 10. Result ： Under certain chromatogram condition, there is linear relation between the camphor and the wintergreen oil and the peak area in the scope of 0. 7785 - 31. 14μg and in 0.7537 - 15.07mg. The average return ratio is 98.35% and 102.725% respectively, RSD 0.6% and 1.20% respectively. This experiment provides an accurate and reliable examination method to guarantee the quality of musk strong bone paste.