小鼠腹腔巨噬细胞的分离培养与鉴定

Separation, Cultivation and Identification of Mouse Peritoneal Macrophages

目的:建立一种简便的巨噬细胞分离与培养方法,为骨替代材料降解产物的研究提供巨噬细胞。方法:以无血清DMEM培养液灌洗小鼠腹腔,10min后吸出灌洗液于含10%胎牛血清的DMEM培养液中培养。采用HE染色和倒置显微镜观察细胞形态;酸性磷酸酶及非特异性酯酶染色测定细胞内酶的表达;墨汁颗粒吞噬实验检测其吞噬功能。结果:获得高纯度的巨噬细胞,具有巨噬细胞的形态特征,良好的吞噬功能,含水解酶类。结论:本法是一种简易而实用的体外分离培养腹腔巨噬细胞的方法。

Objective： To establish a convenient method of separation and cultivation of macrophage. Methods： Abdominal cavity of were douched using DMEM medium without serum, and the contain cells was drawn out and were cultured in DMEM medium with 10% fetal bovine serum. The cells were identified by morphological observation, endocellular enzyme and phagocytosis. Results： The obtained cells were highly purified macrophages with morphologic characteristics of the macrophage in organism, favourable phagocytosis, acid phosphatase （ACP） and α-acetic acid naphthol esterase（α-ANE） activity. Conclusion： This was a simple and pragmatic method for separation and cultivation of mouse peritoneal macrophages.