苯乙酸钠对肺癌A549细胞生长的影响

Effect of Sodium Phenylacetate on Cell Growth of Human Lung Adenocarcinoma Cells

目的:研究抗肿瘤药物苯乙酸钠(NaPA)对体外培养的人肺腺癌细胞株A549抑制率、细胞周期和凋亡的影响。方法:应用四甲基偶氮唑蓝(MTT)比色、流式细胞仪等方法,观察不同浓度NaPA对人肺腺癌A549细胞株生长、细胞周期和凋亡的影响。结果:NaPA对A549有明显的生长抑制作用,且呈剂量时间依赖性,最大抑制率可达79%。NaPA可阻滞A549细胞的细胞周期,使G1/G0期细胞增多,S期细胞减少,同时诱导A549细胞的凋亡,凋亡率最高可达30.5%。结论:苯乙酸钠抑制A549细胞生长,其抑制作用可能是通过NaPA使细胞周期抑制于G1/G0期和诱导细胞凋亡而实现。

Objective： To investigate the effect of sodium phenylacetate （NaPA） on lung adenocarcinoma cell line A549. Methods： Time and dose effects of NaPA were deceted using MTT color metric assay and the influences in the cell cycle and the apoptosis were observed through flow cytometry. Results： NaPA could inhibit the growth of A549 cell line in a dose-depended manner. The biggest rate of cell growth inhibition was up to 79% in the those treated with NaPA. The cytometry confirmed that NaPA could arrest the lung cancer cells from G0/G1 to S stage and induce A549 cells apoptosis. Conclusion： NaPA can inhibit A549 cell line, and the mechanism may be related to the influence in the cell cycle and induction of apoptosis.