体外诱导人骨髓间充质干细胞向胰岛β样细胞分化的研究

In vitro differentiation of human bone marrow mesenchymal stem cells into islet β-like cells

目的:探讨体外诱导人骨髓间充质干细胞(MSCs)向胰岛β样细胞分化。方法:在无菌条件下从正常成人骨髓中分离间充质干细胞,体外培养传3代后用表皮生长因子(epidermal growthfactor,EGF)、β-巯基乙醇和高糖培养基诱导MSCs向胰岛β样细胞分化。观察MSCs在诱导前后的形态变化;用胰岛素免疫细胞化学染色检测胰岛素的表达;用双硫腙染色鉴定胰岛β样细胞。结果:未经诱导的MSCs在培养体系中呈贴壁生长,长梭形,经诱导分化后,细胞逐渐变圆,并聚集成团;胰岛素免疫细胞化学表明细胞团内的细胞呈胰岛素染色强阳性反应;双硫腙染色阳性。结论:人骨髓间充质干细胞可在体外被定向诱导分化为胰岛β样细胞。

Aim： To isohte mesenehymal stem cells （MSCs） from human bone marrow and induce them to differentiate into islet β- like cells. Methods： Mesenchymal stem cells were isolated from human bone marrow and were induced with epidermal growth factor （EGF）,β- mercaptoethanol and high concentration of glucose. Insulin in the treated cells was examined by immunocytochchemistry. The treated cells were stained with dithizone （DTZ）. Results： The uncells were spindle- shaped adherent cells. After induction, MSCs were getting round and formed clusters. Immunocytochemistry confirmed that these clusters were strongly positive for insulin. The clusters were also stain into mauve by DTZ. Conclusion： MSCs from human bone marrow can be induced into islet β- like ceils in vitro.