术后病理性腹腔粘连大鼠模型制作的实验研究

Experimental model of post-operative intra-peritoneal adhesion in rats

目的:研究术后病理性腹腔粘连大鼠模型的制作及运用。方法:用一种尚未见报道的锉刀与干纱布、血管钳、刀片在大鼠蚓突造模,进行大鼠腹腔粘连模型制作的优选。将40只模型SD雄性大鼠(清洁级)随机分为A组(纱布组)、B组(血管钳组)、C组(锉刀组)、D组(刀片组),每组10只,饲养7 d,第8 d处死小鼠,采用Ph illips五级评分法对腹腔粘连进行统计分析。结果:A、B两组造模效果均为Ph illips五级评分标准的Ⅰ级,C组为达Ⅳ级,D组达Ⅲ级,C组与A、B、D组之间两两比较均有显著性差异(P<0.05)。B组病死率为10%,D组达20%,经统计学处理四组间无明显差异(P=0.276 4)。结论:以大鼠的蚓突制作术后病理性腹腔粘连动物模型,锉刀为最佳造模工具,其优点是造模均匀,均达Ⅳ级。

Objective：To set up an animal model of intraabdominal adhesion after surgery. Methods：A new rat model of intraabdominal adhesion was made by using pincers, dry gauze, hemostatic clamps and razor blade. Rat appendix was used to produce intestinal adhesions. Forty male Sprague-Dawley rats were divided into 4 groups： group A（ gauze group）, group B （hemostatic clamp group）, group C （pincer group）, group D（razor blade group）, 10 rats in each group. Animals were fed for 7 days before killed. The extent of adhesion was evaluated by Phillips scale and analyzed statistically. Results： The extents of adhesion in group A and B were grade Ⅰ by Phillips scale, grade Ⅳ in group C, grade Ⅲ in group D. The extent of adhesion in group C was different significantly from group A, B, and D （P 〈 0.05）. The death rate in group B was 10% and 20% in group D, which were not different statistically （P = 0. 2764）. Conclusion： The best way to make a rat model of intraabdominal adhesion is by using rat appendix and rubbed with a pincer, which has the benefit of generalized distribution and extent to grade Ⅳ.