摘要
目的:研究术后病理性腹腔粘连大鼠模型的制作及运用。方法:用一种尚未见报道的锉刀与干纱布、血管钳、刀片在大鼠蚓突造模,进行大鼠腹腔粘连模型制作的优选。将40只模型SD雄性大鼠(清洁级)随机分为A组(纱布组)、B组(血管钳组)、C组(锉刀组)、D组(刀片组),每组10只,饲养7 d,第8 d处死小鼠,采用Ph illips五级评分法对腹腔粘连进行统计分析。结果:A、B两组造模效果均为Ph illips五级评分标准的Ⅰ级,C组为达Ⅳ级,D组达Ⅲ级,C组与A、B、D组之间两两比较均有显著性差异(P<0.05)。B组病死率为10%,D组达20%,经统计学处理四组间无明显差异(P=0.276 4)。结论:以大鼠的蚓突制作术后病理性腹腔粘连动物模型,锉刀为最佳造模工具,其优点是造模均匀,均达Ⅳ级。
Objective:To set up an animal model of intraabdominal adhesion after surgery. Methods:A new rat model of intraabdominal adhesion was made by using pincers, dry gauze, hemostatic clamps and razor blade. Rat appendix was used to produce intestinal adhesions. Forty male Sprague-Dawley rats were divided into 4 groups: group A( gauze group), group B (hemostatic clamp group), group C (pincer group), group D(razor blade group), 10 rats in each group. Animals were fed for 7 days before killed. The extent of adhesion was evaluated by Phillips scale and analyzed statistically. Results: The extents of adhesion in group A and B were grade Ⅰ by Phillips scale, grade Ⅳ in group C, grade Ⅲ in group D. The extent of adhesion in group C was different significantly from group A, B, and D (P 〈 0.05). The death rate in group B was 10% and 20% in group D, which were not different statistically (P = 0. 2764). Conclusion: The best way to make a rat model of intraabdominal adhesion is by using rat appendix and rubbed with a pincer, which has the benefit of generalized distribution and extent to grade Ⅳ.
出处
《医学研究生学报》
CAS
2006年第3期229-231,共3页
Journal of Medical Postgraduates
基金
江苏省教育基金资助项目(批准号:05KJB360094)
江苏省中医药管理基金资助项目(批准号:H05062)
关键词
腹腔粘连
大鼠
模型
锉刀法
Abdominal cavity' s adhesion
Rat
Model
File